Zombi 2 opens with what appears to be an abandoned boat drifting toward New York City. After two curious harbor workers board it, a zombie lunges out of a hidden spot on board and kills one of the two patrolmen. The surviving officer kills the zombie, and the body of the fallen one is taken to a local morgue.
At the morgue, the daughter of the boat owner, Ann Bowles, gets the third degree from local investigators. Ann reveals that the last she heard from her father was that he was working on a research project on a remote island in the Caribbean called Matul. After leaving the morgue, Ann and her colleague Peter West take a boat to the island to find her father.